abstract：:A method for measuring a human immunodeficiency virus (HIV) cell membrane fusion inhibitor (T-20/Ro 29-9800) and its metabolite (M-20/Ro 50-6343) in human plasma by liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. The relatively large peptide analytes and their corresponding deuterated (d(10)) ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Chang D,Kolis SJ,Linderholm KH,Julian TF,Nachi R,Dzerk AM,Lin PP,Lee JW,Bansal SK

更新日期：2005-07-01 00:00:00

abstract：:A method based on capillary zone electrophoresis (CZE) was used to study the interaction between low molecular weight heparin (LMWH) and interleukin 2 (IL-2). The results showed that the increase of the concentration of LMWH led to the decrease of the peak height and the increase of the peak width of IL-2, but the pea...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Liang A,He X,Du Y,Wang K,Fung Y,Lin B

更新日期：2005-07-01 00:00:00

abstract：:This manuscript reports the usefulness of the determination of sulphathiazole (ST) using photochemically induced fluorescence (RTPF) and RTPF coupling with first derivative (D1-RTPF), and the determination of sulphanilamide (SAN) by meaning first derivative of the emission spectrum. By irradiating 5 min, with intense ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： García MC,Diez NM,Gil DB,López FS

更新日期：2005-06-15 00:00:00

abstract：:Three extracts of Calendulae officinalis flos (Asteraceae): heptane, ethyl acetate and methanol were introduced to a human skin fibroblast (HSF) cells culture and a culture of human breast cancer cells (T47D), cell culture collection ECACC number 85102201. The ethyl acetate but not the heptane and methanol extracts in...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Matysik G,Wójciak-Kosior M,Paduch R

更新日期：2005-06-15 00:00:00

abstract：:We describe a method for determining the ambroxol content in tablets nondestructively. To obtain a reliable quantitative calibration, we prepared 20 pellet samples (ambroxol content: 8.30-16.25 wt.%) and acquired their Raman spectra while rotating the pellets. The spectra of the rotated samples reflected the compositi...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Hwang MS,Cho S,Chung H,Woo YA

更新日期：2005-06-15 00:00:00

abstract：:Electrochemical biosensor for the detection of DNA hybridization using the reduction signal of alpha-naphthol is described. A pencil graphite electrode was used as a working electrode. Capture probes were covalently attached on to the pencil graphite electrode surface (PGE) at the 5' end amino group by using N-(dimeth...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kara P,Erdem A,Girousi S,Ozsoz M

更新日期：2005-06-01 00:00:00

abstract：:Ginseng saponins isolated from ginseng, have been regarded as the principal constituents responsible for the biological activities. The aim of this study was to set up a liquid chromatography-mass spectrometry (LC-MS) method for simultaneously determine the concentration of Ginsenoside Rg1 and its secondary glycoside ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Sun J,Wang G,Haitang X,Hao L,Guoyu P,Tucker I

更新日期：2005-06-01 00:00:00

abstract：:A simple and novel method for the extraction and quantification of diltiazem hydrochloride was developed and applied to homogenization and stability studies. The method used solid phase extraction coupled with reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. Validation show...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Buur JL,Baynes RE,Yeatts JL,Davidson G,Defrancesco TC

更新日期：2005-06-01 00:00:00

abstract：:A broad spectrum of sample preparation methods is currently used for the isolation of pharmacologically active compounds from plant and herbal materials. The paper compares the effectiveness of infusion, microwave assisted solvent extraction (MASE), matrix solid-phase dispersion (MSPD) and pressurised liquid extractio...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Dawidowicz AL,Wianowska D

更新日期：2005-04-29 00:00:00

abstract：:Fentanyl, a potent analgesic drug, has traditionally been used intravenously in surgical or diagnostic operations. Formulations with fentanyl in oral transmucosal delivery system and in transdermal depot-patch have also been developed against breakthrough pain in cancer patients. In this report, LC-MS/MS methods to de...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Huynh NH,Tyrefors N,Ekman L,Johansson M

更新日期：2005-04-29 00:00:00

abstract：:6-O-Succinil-beta-cyclodextrin (CDsuc6) was synthesized with very good yield by one pot synthesis and characterized by NMR spectroscopy and ESI-MS. It was used as a chiral selector in capillary electrophoresis to resolve catecholamine racemates, namely norepinephrine, epinephrine, terbutaline and norphenilephrine. The...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Cucinotta V,Giuffrida A,Maccarrone G,Messina M,Puglisi A,Torrisi A,Vecchio G

更新日期：2005-04-29 00:00:00

abstract：:Kumariasava is a marketed ayurvedic formulation containing Aloe vera as one of the main ingredients. Present study aims to standardize Kumariasava based upon chromatographic and spectral studies. Various extracts of Kumariasava have been prepared and evaluated. Chloroform extract indicated presence of three well-resol...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Elamthuruthy AT,Shah CR,Khan TA,Tatke PA,Gabhe SY

更新日期：2005-04-29 00:00:00

abstract：:A combined pH/organic solvent linear gradient mode in high performance liquid chromatography (HPLC) is presented as a new approach to determination of low concentrations of ionogenic analytes in biological material. The approach consists in simultaneous development of linear gradients of pH and organic modifier in the...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Wiczling P,Markuszewski MJ,Kaliszan M,Galer K,Kaliszan R

更新日期：2005-04-29 00:00:00

abstract：:A flow injection spectrophotometric procedure was developed for determination of acetylcysteine in sachets and liquid formulations. The determination of this drug was carried out by reacting it with bromine chemically generated in flow injection system monitored continuously at 400 nm. Acetylcysteine reacts with bromi...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Suarez WT,Vieira HJ,Fatibello-Filho O

更新日期：2005-04-01 00:00:00

abstract：:Diltiazem (DTZ) is an optically active calcium channel blocker having a benzodiazepine structure. The drug used in therapy is (+)-cis-diltiazem with configuration (2S,3S). To describe the analytical profile of DTZ different stationary phases (RP-18, RP-8, monolithic support) were tested. The best separation of DTZ fro...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Quaglia MG,Donati E,Fanali S,Bossù E,Montinaro A,Buiarelli F

更新日期：2005-04-01 00:00:00

abstract：:A method for the separation of polymerized impurities in meropenem has been developed by gel filtration chromatography (GFC). The chromatographic conditions included a Superdex peptide HR 10/30 column and the mobile phase consisting of 0.01 mol L(-1) sodium phosphate buffer (pH 7.0), at a flow rate of 0.8 mL min(-1). ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Cai SY,Hu CQ

更新日期：2005-03-09 00:00:00

abstract：:A procedure based on liquid chromatography-mass spectrometry (LC-MS) is described for determination of caffeine, theobromine, theophylline, taurine in different dietary supplements. After addition of tryptophan as internal standard, both solid and liquid specimens were extracted with 4 ml of hexane/isopropanol (9:1). ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Marchei E,Pellegrini M,Pacifici R,Palmi I,Pichini S

更新日期：2005-03-09 00:00:00

abstract：:We analyzed some glycosaminoglycans and natural and artificial acidic polysaccharides using microchip electrophoresis in the buffer containing ethidium bromide, and found that they were successfully separated and detected within 150 s with comparable sensitivity with that of conventional electrophoresis using cellulos...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Matsuno YK,Kinoshita M,Kakehi K

更新日期：2005-03-09 00:00:00

abstract：:A rapid analytical procedure for routine identification and quantification of amoxicillin in premixes by high performance liquid chromatography was developed and tested. The ground premix samples were extracted for 10 min using 100ml extraction mixture water-methanol (800:200, v/v). The extract was analyzed by reverse...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Dousa M,Hosmanová R

更新日期：2005-02-23 00:00:00

abstract：:A new HPLC method for the determination of puerarin in cerebral ischemia reperfusion rat plasma is introduced. Puerarin, the principal bioactive component of puerariae radix isoflavone, was extracted from plasma by methanol. The HPLC separation was then performed on a reversed-phase C18 column using water-acetonitrile...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Yan B,Xing D,Ding Y,Tao J,Du LJ

更新日期：2005-02-23 00:00:00

abstract：:A direct, extraction-free spectrophotometric assay was developed for determination of angiotensin I-converting enzyme activity (ACE) in the presence of ACE inhibitors using hippuryl-l-histidyl-l-leucine (HHL) as the ACE-specific substrate. This method relies on previously published spectrophotometric determination of ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Li GH,Liu H,Shi YH,Le GW

更新日期：2005-02-23 00:00:00

abstract：:A reverse phase high performance liquid chromatographic method to determine tizanidine (TZ) and rofecoxib (RF) in combination is proposed and applied to the pharmaceuticals. This method allows the determination of 0.1-0.5 microg/ml of TZ and 1.2-6.0 microg/ml of RF along with 10 microg/ml of nimesulide (internal stand...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Gandhimathi M,Ravi TK,Varghese SJ

更新日期：2005-02-07 00:00:00

abstract：:A method for the determination of cocaine (COC) and benzoylecgonine (BZE) in human urine using a column-switching liquid chromatography system is reported. A homemade precolumn (20 mm x 4.6 mm i.d.) dry-packed with Alltech ODS-C18 (35-750 microm) was employed as an extraction precolumn in order to extract and concentr...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Brunetto MR,Delgado Cayama Y,Gutiérrez García L,Gallignani M,Obando MA

更新日期：2005-02-07 00:00:00

abstract：:Two methods are described for the simultaneous determination of tizanidine and rofecoxib in binary mixture. The first method was based on HPTLC separation of the two drugs followed by densitometric measurements of their spots at 311 nm. The separation was carried out on Merck HPTLC aluminium sheets of silica gel 60 F2...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kaul N,Dhaneshwar SR,Agrawal H,Kakad A,Patil B

更新日期：2005-02-07 00:00:00

abstract：:An analytical methodology using square wave voltammetry (SWV) at a hanging mercury drop electrode (HMDE) was developed for the quantitative determination of dipyridamole (DIP), a drug used for the treatment of several cardiovascular diseases, in pharmaceutical tablets and injections of Persantin in phosphate buffer (p...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： de Toledo RA,Castilho M,Mazo LH

更新日期：2005-01-04 00:00:00

abstract：:Quantification of biomarkers can provide important information about the safety and efficacy of candidate drugs. Unfortunately, limited sample volume and excess costs often limit analysis of multiple biomarkers. We developed, optimized, validated, and implemented a multiplex immunoassay for simultaneous measurement of...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Ray CA,Bowsher RR,Smith WC,Devanarayan V,Willey MB,Brandt JT,Dean RA

更新日期：2005-01-04 00:00:00

abstract：:In the framework of a preliminary investigation on the plasma profile of cloxacillin after oral administration, a simple and rapid LC method was developed for the direct determination of this compound in human plasma. The on-line sample clean-up was carried out using a weak anion exchanger (diethylaminoethyl groups) a...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Rbeida O,Chiap P,Lubda D,Boos KS,Crommen J,Hubert P

更新日期：2005-01-04 00:00:00

abstract：:The interaction between monoammonium glycyrrhizinate (MAG) and bovine serum albumin (BSA) were studied by fluorescence and absorption spectroscopy. The quenching mechanism of fluorescence of bovine serum albumin by monoammonium glycyrrhizinate was discussed. The binding sites number n and apparent binding constant K w...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Hu YJ,Liu Y,Wang JB,Xiao XH,Qu SS

更新日期：2004-11-19 00:00:00

abstract：:A near-infrared reflection spectroscopy (NIRS) method was developed to determine the total content of kavapyrones, kavain and water in dry extracts of Piper methysticum Forst. (kava kava, Piperaceae). Based on the recorded spectra and the reference data, performed by HPLC and Karl Fischer titration, a chemometrical an...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Gaub M,Roeseler Ch,Roos G,Kovar KA

更新日期：2004-11-19 00:00:00

abstract：:Pharmaceuticals are produced according to current pharmacopoeias, which require quality parameters. Tablets of identical formulation, produced by different factories should have the same properties before and after storage. In this article, we analyzed samples having two different origins before and after storage (30 ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Roggo Y,Roeseler C,Ulmschneider M

更新日期：2004-11-19 00:00:00

abstract：:The present manuscript deals with a simple and sensitive flow-injection method for the chemiluminescent determination of thiazides. The method is based on the on-line photodegradation and chemiluminescent determination of the resulting photo-fragments. The on-line photodegradation is performed in basic medium by using...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Ciborowski M,Icardo MC,Mateo JV,Martínez Calatayud J

更新日期：2004-11-19 00:00:00

abstract：:A novel and quick high-performance liquid chromatography (HPLC) method with UV spectrophotometric detection was developed and validated for the determination of five compounds in topical gel. The described method is suitable for simultaneous determination of active component ketoprofen, two preservatives methylparaben...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Dvorák J,Hájková R,Matysová L,Nováková L,Koupparis MA,Solich P

更新日期：2004-11-15 00:00:00

abstract：:The crystallographic texture, i.e. the preferred orientation of crystallites of delta-mannitol samples, has been experimentally determined by pole figure analysis. The pole figures were measured with an X-ray diffraction texture goniometer. It was found that already the uncompressed delta-mannitol powder sample was sl...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Koivisto M,Virjonen T,Heikkilä T,Lehto VP

更新日期：2004-11-15 00:00:00

abstract：:The water-soluble vitamins, pyridoxine, riboflavin, and thiamine, were separated by reversed-phase liquid chromatography using hot or superheated water as the mobile phase and were detected using a range of detectors, including ultraviolet and fluorescence spectroscopy and mass spectrometry. By using deuterium oxide a...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Chienthavorn O,Smith RM,Saha S,Wilson ID,Wright B,Taylor SD,Lenz EM

更新日期：2004-11-15 00:00:00

abstract：:A simple and rapid method for determination of icariin (ICA) and its two metabolites, icaritin (ICT) and desmethylicaritin (DICT), by capillary zone electrophoresis has been developed and validated. Optimum separation of ICA, ICT, and DICT was obtained on 43.6 cm x 50 microm capillary using sodium tetraborate (30 mmol...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Liu J,Lou YJ

更新日期：2004-10-29 00:00:00

abstract：:The purpose of this work was to demonstrate the possibilities offered by laser-induced breakdown spectroscopy (LIBS) for the direct and rapid analysis of pharmaceutical liquid formulations. Sodium chloride in solution was chosen as a model compound. A pulsed Nd:YAG laser (1064 nm) was used to produce a gaseous plasma ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： St-Onge L,Kwong E,Sabsabi M,Vadas EB

更新日期：2004-10-29 00:00:00

abstract：:This paper puts forward a method to describe an equation of the within-plate uncertainty (relative standard deviation (R.S.D.) of measurements) as a function of analyte concentration in sandwich enzyme-linked immunosorbent assay (ELISA). A kit for thyroid stimulating hormone is taken as an example. The pipetting proce...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Hayashi Y,Matsuda R,Maitani T,Ito K,Nishimura W,Imai K,Maeda M

更新日期：2004-09-21 00:00:00

abstract：:A polarographic study about how three anti-inflammatories, such as Aceclofenac, Tenoxicam and Droxicam behave, using tast polarography (TP) and differential pulse polarography (DPP) was carried out. These studies were always carried out in a media formed by Methanol-Britton-Robinson aqueous buffer (0.1M) (4:96 (v/v)) ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Acuña JA,Vázquez MD,Tascón ML,Sánchez-Batanero P

更新日期：2004-09-21 00:00:00

abstract：:The equivalent response of evaporative light scattering detector (ELSD) for compounds of similar structure is exploited to develop an LC/ELSD method for the simultaneous quantitation of the four main components of gentamicin sulfate, using as external standard the one main component kanamycin. A C18 column was used al...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Megoulas NC,Koupparis MA

更新日期：2004-09-21 00:00:00

abstract：:In order to develop a radiopharmaceutical for hepatobiliary scintigraphy with better hepatobiliary properties new ligand for complexation of 99mTc, 4-iodo-2,6-dimethylphenylcarbamoylmethyl iminodiacetic acid (METHYLIODIDA), was synthesized. Acid-base equilibria of METHYLIODIDA were studied potentiometrically, because ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Jovanović MS,Popović G,Kapetanović V,Orlić M,Vladimirov S

更新日期：2004-09-03 00:00:00